Products > Genial > Anti-Clotting Reagent

Anti-Clotting Reagent Product Code: GGS-JL001 Number of tests per Unit: 10 - 20 assays per reconstituted vial. Makes 100ml and is used as 10mls per sample. Description: Anti-clotting Reagent (ACR) is an enzyme mixture which when reconstituted helps rapidly breakdown the protein components (fibrin) of blood clots which can cause culture problems, particularly with amniotic and bone marrow samples. How it works: Since specimens often arrive in non-heparinized containers, the addition of ACR to the sample rapidly breaks down blood clots. This preserves valuable amniotic and bone marrow samples and thus eliminates the need for repeat sampling. How to use it: The user must dissolve the dried ACR reagent in the stated amount of PBS and then filter it using a micro-syringe filter. The filtered solution can be aliquoted and frozen for future use. Two suggested protocols are provided in Package Insert with the general purpose being the rapid dissolution (5 - 10 minutes) of blood clots in the sample. In general, it has been found that the lab should use 10ml of ACR for Bone Marrow and Peripheral Blood (PB) samples while 5ml of ACR should be used for CVS samples. Benefit to Lab: The product contains no streptokinase (another blood clot dissolver), which is known to have cytotoxic effects. Since blood clots hinder the cell growth of the required cell types, the use of Anti-clotting Reagent allows for more rapid establishment of cell colonies with resultant quicker harvest times for the sample. Additional Information: Refer to Case Study 1

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